Crack |WORK| Schemaplic V 3.0
Crack |WORK| Schemaplic V 3.0
Crack Schemaplic V 3.0
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Acetylcholine-stimulated phosphoinositide hydrolysis in response to stimulation of muscarinic receptors in rat cortical slices.
Rats were anesthetized with urethane. The muscarinic receptor subtype(s) coupled to phosphoinositide hydrolysis was studied in slices of rat cortical tissue containing the somatosensory and temporal cortex. The concentration of inositol phosphates (IPs) was measured in the presence of lithium. The muscarinic receptor antagonist atropine reversibly decreased the stimulation of IP accumulation that was produced by carbachol, an agonist at M1 and M2 muscarinic receptors. In contrast, the muscarinic receptor agonist oxotremorine-M, an M4 agonist, increased phosphoinositide hydrolysis. Phencyclidine, a non-competitive receptor antagonist at NMDA receptors, reversibly reduced the elevation in IP accumulation caused by carbachol and oxotremorine-M. The results of this study provide evidence that phosphoinositide hydrolysis in neurons is a possible mechanism for enhancing calcium influx that is mediated by M1 and M2 muscarinic receptors. In addition, the stimulation of phosphoinositide hydrolysis by oxotremorine-M in the somatosensory and temporal cortex suggests a novel pathway for acetylcholine that involves the activation of M4 receptors./* -*- Mode: C++; tab-width: 2; indent-tabs-mode: nil; c-basic-offset: 2 -*- */
/* ***** BEGIN LICENSE BLOCK *****
* Version: MPL 1.1/GPL 2.0/LGPL 2.1
* The contents of this file are subject to the Mozilla Public License Version
* 1.1 (the "License"); you may not use this file except in compliance with
* the License. You may obtain a copy of the
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